Philip Cocu, Wesley Sneijder and Giovanni van Bronckhorst suffered injuries during the World Cup warm-up on Sunday against Australia.
Van Bronckhorst was hurt by a challenge from Luke Wilkshire, who was sent off after receiving his second yellow card.
Sneijder's injury was described as serious by Dutch coach Marco van Basten .
'Sneijder is limping and his ankle is strapped up,' van Basten told reporters.
Van Basten will announce Wednesday if there will be a need for substitutes in the team that will start next Sunday with Serbia and Montenegro, probably there will be a player from the under-21 squad, if any.